E. coli Source Tracking by 
Rep-PCR DNA Fingerprinting
 
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Source Database Summary

Table 1. E. coli isolates correctly classified into thirteen source groups using the fluorophore-enhanced DNA fingerprinting (HFERP) method.

Source group Individuals Number of DNA Fingerprints
Cat 37 48
Chicken 86 144
Cow 115 189
Deer 64 96
Dog 71 106
Duck 42 81
Goat 36 42
Goose 73 135
Horse 44 78
Human 197 210
Pig 111 215
Sheep 37 61
Turkey 69 126
Overall 982 1531



Table 2. Total and unique E. coli isolates correctly classified into source groups by rep-PCR and HFERP DNA fingerprinting methodsa.

Source group All Fingerprints (n=2,466) Unique Fingerprints (n=1,535)
Percent Correctly Classifieda Percent Correctly Classified
rep-PCR rep-PCR HFERP
Pearson Pearson Jaccard Pearson Jaccard
Petb 91.8 (279)d 61.7 (95)d 45.5 (70) 59.1 (91) 44.8 (69)
Chicken 81.4 (188) 59.7 (86) 38.9 (56) 63.2 (91) 31.9 (46)
Cow 79.6 (238) 55.0 (104) 47.6 (90) 62.0 (117) 48.2 (91)
Deer 85.5 (145) 55.2 (53) 36.5 (35) 62.2 (60) 42.6 (41)
Waterfowlc 81.4 (262) 66.2 (150) 52.8 (114) 70.4 (152) 56.5 (122)
Goat 97.1 (101) 66.7 (27) 59.5 (25) 47.6 (20) 42.9 (18)
Horse 69.3 (79) 44.3 (35) 34.2(27) 52.6 (41) 32.1 (25)
Human 78.3 (240) 59.2 (124) 47.4(100) 53.8 (113) 45.2 (95)
Pig 77.9 (236) 63.7 (137) 43.7 (94) 54.4 (117) 36.3 (78)
Sheep 79.0 (80) 7.5 (29) 39.3 (24) 37.7 (23) 8.2 (5)
Turkey 88.6 (179) 73.8 (93) 52.4 (66) 73.0 (92) 54.8 (69)
Overall 82.2 (2,027) 60.9 (933) 45.8 (701) 59.9 (917) 43.0 (659)

aBased on Jackknife analysis with 1% optimization and maximum similarities using curve-based (Pearson's product moment correlation coefficient) or band-based (Jaccard's coefficient) similarity calculations.
bPet group consists of cats and dogs.
cWaterfowl group consists of ducks and geese.
dValues in parentheses are number of isolates correctly classified.